Abstract
Potato (Solanum tuberosum L.) is the third most important crop after rice and wheat in the world and it is one of the most efficient food crops producing more yield, alimental fibre, high-quality protein, minerals and vitamins than wheat, maize and rice per unit area and time and is recognized as a steady and healthy food crop. The production of seed potatoes and its supply chain face special problems because they are big in size, multiply slowly and large quantities are needed to plant the next crop. There is a huge gap between production and supply of quality planting materials of potato. So, it is important to develop a systematic protocol for production of planting materials at large scale. Given such conditions, the conception of an effective and reproducible in vitro regeneration system in popular potato cultivar(s) is crucial to produce high-quality planting material at large scale. Keeping this in mind, to produce genuine propagative material of high phytosanitary, physiological and genetic quality, effective and reproducible in vitro regeneration protocols of potato are indispensable. To attain such goals, potato sprouts were employed as explants sources and inoculated on MS medium fortified with different auxins and cytokinins in fluctuating proportions or alone as well as in diverse concentrations and amalgamations in combination with 30.0 g l−1 sucrose and 7.5 g l−1 agar. Induction medium MS3NB (MS + 3.0 mg l−1 NAA + 1.0 mg l−1 BA) was more responsive for callus induction (83.00%). Whilst nutrient media MS3BN (MS + 3.0 mg l−1 BAP + 1.0 mg l−1 NAA) displayed better in vitro competence, i.e. number(s) of shoot inducing explants (91.00%) and number(s) of shoot(s) per explant (6.50) along with shoot of bigger length (4.60). Whilst higher in vitro rooting response, viz., root proliferating efficiency (84.0%), number(s) of roots (9.0) with higher length (5.1) was exhibited by rooting medium MSIB (MS + 1.0 mg l−1 IBA). For inducing rooting in vitro, a reduced level of sucrose, i.e. 15.0 g l−l, was used. The regenerants were shifted to pots and acclimatized in polyhouse/net house during preliminary ablactating phase. Regenerants showed normal growth and morphology and were found efficacious in the external environment after hardening. Culture medium with higher regeneration aptitude obtained in present investigation may be used for mass in vitro propagation, micro tuber production and different biotechnological works including tailoring transgenic plants resistant/tolerant against diverse biotic and abiotic stresses.
Abstract
Potato (Solanum tuberosum L.) is the third most important crop after rice and wheat in the world and it is one of the most efficient food crops producing more yield, alimental fibre, high-quality protein, minerals and vitamins than wheat, maize and rice per unit area and time and is recognized as a steady and healthy food crop. The production of seed potatoes and its supply chain face special problems because they are big in size, multiply slowly and large quantities are needed to plant the next crop. There is a huge gap between production and supply of quality planting materials of potato. So, it is important to develop a systematic protocol for production of planting materials at large scale. Given such conditions, the conception of an effective and reproducible in vitro regeneration system in popular potato cultivar(s) is crucial to produce high-quality planting material at large scale. Keeping this in mind, to produce genuine propagative material of high phytosanitary, physiological and genetic quality, effective and reproducible in vitro regeneration protocols of potato are indispensable. To attain such goals, potato sprouts were employed as explants sources and inoculated on MS medium fortified with different auxins and cytokinins in fluctuating proportions or alone as well as in diverse concentrations and amalgamations in combination with 30.0 g l−1 sucrose and 7.5 g l−1 agar. Induction medium MS3NB (MS + 3.0 mg l−1 NAA + 1.0 mg l−1 BA) was more responsive for callus induction (83.00%). Whilst nutrient media MS3BN (MS + 3.0 mg l−1 BAP + 1.0 mg l−1 NAA) displayed better in vitro competence, i.e. number(s) of shoot inducing explants (91.00%) and number(s) of shoot(s) per explant (6.50) along with shoot of bigger length (4.60). Whilst higher in vitro rooting response, viz., root proliferating efficiency (84.0%), number(s) of roots (9.0) with higher length (5.1) was exhibited by rooting medium MSIB (MS + 1.0 mg l−1 IBA). For inducing rooting in vitro, a reduced level of sucrose, i.e. 15.0 g l−l, was used. The regenerants were shifted to pots and acclimatized in polyhouse/net house during preliminary ablactating phase. Regenerants showed normal growth and morphology and were found efficacious in the external environment after hardening. Culture medium with higher regeneration aptitude obtained in present investigation may be used for mass in vitro propagation, micro tuber production and different biotechnological works including tailoring transgenic plants resistant/tolerant against diverse biotic and abiotic stresses. Leer más
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