In this study, the Anoectochilus roxburghii (A. roxburghii) was studied for its chemical composition and biological activities. The first aim of this work was to isolate and purify compounds from A. roxburghii using Sephadex LH-20 and HPLC. The identification of seven compounds was achieved, with 5,4′-dihydroxy-3,3′,7-trimethoxy-flavone and 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one being isolated from A. roxburghii for the first time. The second aim was to describe its inhibition on the metabolism-related enzyme activities in vitro. Further, 3,5-dihydroxy-7,3′,4′-trimethoxyflavone and 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one showed inhibitory activity on xanthine oxidase, while isorhamnetin-3-O-rutinoside (IC50 = 87.45 μg/mL) may be potential inhibitors of α-amylase. Molecular docking analysis revealed that isorhamnetin-3-O-rutinoside exhibited the highest binding affinity towards PL. In addition to scientifically expanding the compound library of A. roxburghii, the discovery is also a vital reference for finding new insights in the field of medication research, especially as natural metabolism-related enzyme inhibitors.
Journal of Food Biochemistry, Volume 2024, Issue 1, 2024. Read More
Related posts:
Dietary Oryzanol (Ory) Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Activities of Digestive Enzymes, Antioxidant Capacity, and Lipid Metabolism
Laminarin ameliorates alcohol‐induced liver damage and its molecular mechanism in mice
Effects of Tannin Supplementation in Diet on the Resistance to Ammonia Stress of Pacific White Shrimp Litopenaeus vannamei
Effects of Phytase and Microalgae Supplementation on the Utilization of Aquafeeds for European Seabass (Dicentrarchus labrax) Formulated with a High Inclusion Level of Plant Protein
Network Pharmacology Prediction and Metabolomics Validation of the Novel Targets of Morus alba L. against High‐Fat Diet‐Induced Diabetes Mellitus in C57/6J Mice
Beneficial role of Boehmeria nivea in health and phytochemical constituents