Curcuminoids are natural products with widespread biological activity mostly extracted from Curcuma longa and its family. In the current study, we report the first time to isolate curcuminoids from another phyto source. Three compounds (curcumin, desmethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC)) were isolated from the plant Deverra tortuosa using methanolic extract, which was then fractionated and separated by puriFlash. The purity of compounds was monitored by thin-layer chromatography and confirmed by high-performance liquid chromatography. The mass spectrometry identified the isolated compound by their electrospray ionisation. Molecular docking then demonstrated that curcumin and BDMC bind at the same cavity (187 Å) on SARS-CoV-2 Mpro protein indicating similarities while DMC binds to a cavity with a larger size (372 Å). Among the three curcuminoids, BDMC was shown to give the highest binding energy (−7.5 kcal/mol) followed by curcumin (−7.4 kcal/mol) and DMC (−7.3 kcal/mol). Calculating the half-maximal cytotoxic concentration and inhibitory median concentration of both DMC and BDMC showed low-to-moderate antiviral activity against SARS-CoV-2.
Journal of Food Biochemistry, Volume 2024, Issue 1, 2024. Read More
Related posts:
Beneficial role of Boehmeria nivea in health and phytochemical constituents
Chemical Composition and In Vitro and In Silico Biological Activities of Myrciaria tenella (DC.) O.Berg (Myrtaceae) Essential Oil from Brazil
Network Pharmacology Prediction and Metabolomics Validation of the Novel Targets of Morus alba L. against High‐Fat Diet‐Induced Diabetes Mellitus in C57/6J Mice
Phenolic Screening and Mixture Design Optimization for In Vitro Assessment of Antioxidant and Antimicrobial Activities of Honey, Propolis, and Bee Pollen
Rapid and Visual Favor Analysis Using Gas Chromatography‐Ion Mobility Spectrometry (GC‐IMS) in Meat Products: Research Progress and Future Trends
Wheat bran arabinoxylans: Chemical structure, extraction, properties, health benefits, and uses in foods